I have long been interested in microscopy, and am slowly putting together a varied collection of instruments.
MICROSCOPESSome of the photomicrographs shown on these pages were taken through a Watson "Bactil" binocular/monocular (convertible) microscope of about 1954 (the same age as myself!), fitted with a quadruple nosepiece. The picture below shows the monocular version, which is most convenient for photomicrography. I also use an Olympus FH, a Projectina and a Wild M8. My latest acquisition is a Zeiss GFL. To see photos of my microscopes, click on the image above ... and can anyone tell me anything about this camera ??
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TIPS & TECHNIQUESLinks to pages with useful (hopefully!) information Flash PhotomicrographyHow I use flash with my photomicrographic setup Home Made Phase ContrastPhase contrast objectives can be expensive, so wouldn't it be nice to make one's own. Of course you could try etching your own phase plates, but hydrofluoric acid is not nice stuff. An alternative is to use a phase plate of plain glass with a sooted pattern on the plate. Here is an article describing the technique, with links to transcripts of original references from 1953: Immobilising SpecimensTry a drop of lidocaine throat spray -better than benzocaine because benzocaine is not very water soluble, and crystallises, meaning specimens then have to be washed. Aquarium Slides |
I started out using an old Kodak Instamatic 33 adapted to fit the microscope. With this, it is necessary to produce the image at infinity, (I used to project the image onto the ceiling!) before attaching the camera, as the camera lens is still in place and there is no through the lens viewfinder on the camera. Essentially, it is used as a film holder with a shutter. Exposure is by judgement and a little luck! Most of the pictures on this site, however, have been taken with a digital camera, which is a far easier option. Here is a page on how I attach my camera to my various microscopes. Also how I use flash with my photomicrographic setup.
I would like to emphasise that the simplest photographic equipment can produce good results. It is the microscope that does the work!
Not to be confused with Photomicrography, this is the art of making very small photographs, usually on a microscope slide. This was a popular novelty in the 19th Century. Here is my collection of microphotographs